pp7 mcherry plasmids  (Addgene inc)

Journal: STAR Protocols

Article Title: Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO

doi: 10.1016/j.xpro.2022.101630

Figure Lengend Snippet: SunRISER plasmid variants

Article Snippet: cmv-mCherry-10×PP7 (detection plasmid) , ( ) , Addgene #185796.

Techniques: Plasmid Preparation

Journal: STAR Protocols

Article Title: Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO

doi: 10.1016/j.xpro.2022.101630

Figure Lengend Snippet:

Article Snippet: cmv-mCherry-10×PP7 (detection plasmid) , ( ) , Addgene #185796.

Techniques: Recombinant, Plasmid Preparation, Software

Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet:

Article Snippet: pDZ645 pKAN 1x-mCherry-12×PP7 V4 , ( ) , Addgene #73173.

Techniques: Recombinant, Software

Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet:

Article Snippet: Ubc-nls-PCP-12×MoonTag is made by using 12×MoonTag-12×SunTag-kif18b-24×PP7 (Addgene #128606 ( )) as backbone and removing sequences after 12×MoonTag and inserting stop codon and inserting ubc-nls-PCP fragment between SpeI and HindIII sites in front of 12×MoonTag. cmv-mCherry-12×PP7 was constructed by inserting cmv promoter between NotI and BamHI sites into pDZ645 pKAN 1×-mCherry-12×PP7 V4 (Addgene #73173 ( )).

Techniques: Recombinant, Software